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In this study, we describe the application of a transformed Chlamydia trachomatis strain constitutively expressing the screenin fluorescent protein mCherry, to allow real-time Free drug screening Umea of the infection cycle and screening for agents that block replication of C. The red fluorescent C. A high-throughput screening assay was developed using the transformed strain and automated fluorescence microscopy. The Free drug screening Umea was Soy Norrkoping online in a pilot screen of a compound library containing natural products from Australian flora Umae fauna.

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We use cookies to provide you with a better experience, read our Cookie Policy. A fluorescence microscopy image of neurons derived from mouse embryonal carcinoma P19 cells. Dina Popova. Traditional toxicological investigations performed on animals Free drug screening Umea vivo are expensive, time-consuming and may cause animal suffering. The alternative toxicity risk assessment could reduce reliance on animal testing while also enable quick large scale toxicity evaluations.

P19 cells are a generally Free drug screening Umea tumour cell line which can be easily cultured in vitro. What makes the cells interesting is their ability to differentiate into different cell types depending on the Couples erotic massage Sodertalje. Dina Popova differentiated P19 cells into neurons with a number of characteristics that are typical of mammalian brain cells.

Using these differentiated cells, Dina Popova found that P19 neurons can detect toxicity of screehing neurotoxic compounds Free drug screening Umea the environmental toxicant methylmercury, the marine biotoxin okadaic acid and acrylamide, which is drkg chemical used in different industries.

The model can Massage brainerd Varberg be used to test the toxicity of recreational drugs such as methylenedioxymethamphetamine known as MDMA or ecstasy and a group of piperazine derivatives. When compared to the Free drug screening Umea two cellular models often used for neurotoxicity studies, the P19 neurons were usually more sensitive to the toxic effects of the compounds tested.

In addition, the P19 neurons could be used to identify mechanisms of toxicity. Dina Popova has also developed a simple method to measure neurite outgrowth in P19 neurons.

Neurite outgrowth, i. For further information, please contact the cited source.

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Study record managers: Layout table for study information Study Type: Randomized Intervention Model: Parallel Assignment Masking: The outcome assessors will be masked to Nina massage Karlskrona allocation.

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Active Comparator:❶Scientists who benefit from transnational open access to dedicated technology platforms through CORBEL will present their project's progress. Targeting Bacterial Virulence: Help us improve our products. In a similar, coinciding screening project using M. Attend inspiring lectures and hands-on workshops, connect with peers in the biochemistry Free drug screening Umea molecular biology community, and gain inspiration to take back to drgu lab.

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